Reagent Preparation: Dilute MOG peptide in PBS (10mg/ml; stock): MOG35-55 peptide is diluted in PBS to make a stock solution at a concentration of 10mg/ml. 200ug of this solution (20ul) will be used for each mouse. Store at -70°C. Mix M. tuberculosis with CFA: Mix 100mg of M. tuberculosis with 10ml of Complete Freund's Adjuvant (CFA) to get a concentration of 10mg/ml. Store at 4°C. Dilute Pertus..
