1. removed media (centrifuge 4℃로 미리 맞춰준다) 2. PBS wash X 2 (volume 2ml 정도) 3. add TRIZOL to dish (1㎖) 4. stand at RT for 5min, scrap and transfer to E. tube 5. add 0.2㎖ of chloroform 6. vortex for 15sec and stand at RT for 2~3min 7. spin down ×12000g at 4℃ for 15min 8. Transfer the aqueous phase(60% of initiate TRIZOL) to new tube 9. add 0.5㎖ of Isopropanol and at RT for 10min (inverting) 10. ×12..
