DAY 1: Preparation and Primary Antibody Application 60 ℃ incubation overnight slide Xylene – 5 min (deparaffinⅠ) , 5 min (deparaffin Ⅱ), 10 min (deparaffin Ⅲ) 100% EtOH – 2 min x 1 time 95% EtOH – 2 min x 2 times Rinse in water – 5 min (wash in running water for 5 minutes in a bucket) Microwave 100 ℃ with citrate buffer in the chamber – (immersion of slide in boiling buffer) Cool down – 30 min (..
